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Renca
Renca
規(guī)格:
貨期:
編號:B167192
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Renca
商品貨號 B167192
Organism Mus musculus, mouse
Tissue kidney
Cell Type epithelial
Product Format frozen
Morphology epithelial-like
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease renal adenocarcinoma
Age 6 weeks
Gender male
Storage Conditions liquid nitrogen vapor phase
Images
Derivation
The Renca cell line was derived from a tumor that arose spontaneously as a renal cortical adenocarcinoma in Balb/cCr mice.
Clinical Data
male
Tumorigenic YES
Comments
The pattern of growth of this tumor accurately mimics that of human adult renal cell carcinoma, particularly with regard to spontaneous metastasis to lung and liver.?RefMurphy GP, Hrushesky WJ. A murine renal cell carcinoma. J. Natl. Cancer Inst. 50(4):1013-25, 1973. PubMed: 4703766?RefSalup RR, et al. Role of natural killer activity in development of spontaneous metastases in murine renal cancer. J. Urol. 134(6):1236-41, 1985. PubMed: 4057425?The cells do not express transforming growth factor-beta type II receptor (TbetaR-II) 10414746.?RefEngel J, et al. Transforming growth factor-beta type II receptor confers tumor suppressor activity in murine renal carcinoma (renca) cells . Urology. 54(1):164-70, 1999. PubMed: 10414746
Complete Growth Medium

The base medium for this cell line is RPMI-1640 (ATCC 30-2001). To make the complete growth medium, add the following components to the base medium: 

  • 10% Fetal Bovine Serum (BSA; ATCC 30-2020)
  • Non-essential amino acids (NEAA; 0.1mM extra)
  • Additional sodium pyruvate (1 mM extra)
  • Additional L-glutamine (2 mM extra)

This medium is formulated for use with a 5% CO2 in air atmosphere.
Subculturing
Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 2 X 104 to 4 X 104 viable cells/cm2 is recommended.
  6. Incubate cultures at 37°C. We recommend that you subculture when the culture reaches a cell concentration between 8 X 104 and 1.5 X 105 cells/cm2.

Subcultivation ratio: A subcultivation ratio of 1:4 to 1:10 is recommended.
Medium renewal: Every 2 to 3 days.

Cryopreservation
Freeze medium: RPMI-1640 Medium, 77.5%; FBS, 15% FBS; DMSO, 7.5%
Storage temperature liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Population Doubling Time approximately 24 hours
Name of Depositor R Wiltrout
Year of Origin 1969
References

Murphy GP, Hrushesky WJ. A murine renal cell carcinoma. J. Natl. Cancer Inst. 50(4):1013-25, 1973. PubMed: 4703766

Salup RR, et al. Role of natural killer activity in development of spontaneous metastases in murine renal cancer. J. Urol. 134(6):1236-41, 1985. PubMed: 4057425

Engel J, et al. Transforming growth factor-beta type II receptor confers tumor suppressor activity in murine renal carcinoma (renca) cells . Urology. 54(1):164-70, 1999. PubMed: 10414746

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