| 產品名稱 | WiDr |
|---|---|
| 商品貨號 | B165948 |
| Organism | Homo sapiens, human |
| Tissue | colon |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | colorectal adenocarcinoma |
| Gender | female |
| Applications | transfection host |
| Images |  |
| Derivation | Although deposited with the ATCC as a colon adenocarcinoma line established from a 78 year old female, DNA fingerprinting has shown this line to be a derivative of HT-29 (ATCC HTB-38). - |
| Clinical Data | Although deposited with the ATCC as a colon adenocarcinoma line established from a 78 year old female, DNA fingerprinting has shown this line to be a derivative of HT-29 (ATCC HTB-38). female |
| Antigen Expression | HLA A24, A32, B15, B18 |
| Receptor Expression | epidermal growth factor (EGF) |
| Genes Expressed | - carcinoembryonic antigen (CEA) 118 ng/10 exp6 cells/10 days; Colon Specific Antigen (CSAp); transforming growth factor beta; keratin,HLA A24, A32, B15, B18. The cells are positive for keratin by immunoperoxidase staining. The cells expressed p53 antigen (the p53 produced has a G -> A mutation resulting in Arg -> His at position 273). |
| Cellular Products | carcinoembryonic antigen (CEA) 118 ng/10 exp6 cells/10 days; Colon Specific Antigen (CSAp); transforming growth factor beta; keratin |
| Tumorigenic | Yes |
| Effects | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| Comments | Although deposited with the ATCC as a colon adenocarcinoma line established from a 78 year old female, DNA fingerprinting has shown this line to be a derivative of HT-29 (ATCC HTB-38). The cells are negative for Colon Antigen 3 expression. The cells are positive for keratin by immunoperoxidase staining. The cells expressed p53 antigen (the p53 produced has a G -> A mutation resulting in Arg -> His at position 273). Growth of WiDr cells is inhibited by tumor necrosis factor alpha (TNF alpha). Inhibitors of dihydrofolate reductase are highly cytotoxic to WiDr cells. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:20 is recommended
Medium Renewal: Twice per week
|
| Cryopreservation | culture medium 95%; DMSO, 5% |
| Culture Conditions | Temperature: 37.0°C |
| STR Profile | Amelogenin: X CSF1PO: 11,12 D13S317: 11,12 D16S539: 11,12 D5S818: 11,12 D7S820: 10 THO1: 6,9 TPOX: 8,9 vWA: 17,19 |
| Isoenzymes | ES-D, 1 G6PD, B PEP-D, 1 PGD, A PGM1, 1-2 PGM3, 1-2 |
| Name of Depositor | P Noguchi |
| Deposited As | Homo sapiens |
| References | Noguchi P, et al. Characterization of WiDr: a human colon carcinoma cell line. In Vitro 15: 401-408, 1979. PubMed: 90012 Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111 Chen TR, et al. WiDr is a derivative of another colon adenocarcinoma cell line, HT-29. Cancer Genet. Cytogenet. 27: 125-134, 1987. PubMed: 3472642 Smith SG, et al. Cytotoxicity of antifolate inhibitors of thymidylate and purine synthesis to WiDr colonic carcinoma cells. Cancer Res. 53: 5697-5706, 1993. PubMed: 8242626 Rodrigues NR, et al. p53 mutations in colorectal cancer. Proc. Natl. Acad. Sci. USA 87: 7555-7559, 1990. PubMed: 1699228 Petricciani JC, et al. A comparison of three in vivo assays for cell tumorigenicity. Cancer Res. 34: 105-108, 1974. PubMed: 4203458 Tsuboi KK, Kwong LK. Antiproliferative agents and differential survival between normal and cancer cells. Cancer Res. 38: 3745-3750, 1978. PubMed: 698934 Noguchi PD, et al. Chick embryonic skin as a rapid organ culture assay for cellular neoplasia. Science 199: 980-983, 1978. PubMed: 203036 Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066 Olive PL, Banath JP. Multicell spheroid response to drugs predicted with the comet assay. Cancer Res. 57: 5528-5533, 1997. PubMed: 9407963 Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
