| 產品名稱 |
UC10-4F10-11 |
| 商品貨號 |
B165903 |
| Organism |
hamster (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The UC10-4F10-11 hybridoma cell line was produced by fusing Sp2/0-Ag14 myeloma cells with spleen cells from an Armenian hamster that had been immunized with the extracellular portion of of murine CTLA-4 fused to a murine IgG2a. The antibody can be used to detect and quantitate CTLA-4 expression by flow cytometric analysis, and can be used to immunoprecipitate the antigen from CTLA-4 positive cells. Under conditions of suboptimal T cell receptor (TCR) stimulation, whole antibody or Fab fragments from 4F10 can augment T cell proliferation in allogeneic mixed lymphocyte reactions. However, when optimal TCR stimulation is present, 4F10 can suppress T cell proliferation. |
| Derivation |
The UC10-4F10-11 hybridoma cell line was produced by fusing Sp2/0-Ag14 myeloma cells with spleen cells from an Armenian hamster that had been immunized with the extracellular portion of of murine CTLA-4 fused to a murine IgG2a. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse CTLA-4 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse CTLA-4 |
| Comments |
The UC10-4F10-11 hybridoma cell line was produced by fusing Sp2/0-Ag14 myeloma cells with spleen cells from an Armenian hamster that had been immunized with the extracellular portion of of murine CTLA-4 fused to a murine IgG2a. The antibody can be used to detect and quantitate CTLA-4 expression by flow cytometric analysis, and can be used to immunoprecipitate the antigen from CTLA-4 positive cells. Under conditions of suboptimal T cell receptor (TCR) stimulation, whole antibody or Fab fragments from 4F10 can augment T cell proliferation in allogeneic mixed lymphocyte reactions. However, when optimal TCR stimulation is present, 4F10 can suppress T cell proliferation. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.01 mM nonessential amino acids and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
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| Subculturing |
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG |
| Name of Depositor |
JA Bluestone |
| References |
Walunas TL, et al. CTLA-4 can function as a negative regulator of T cell activation. Immunity 1: 405-413, 1994. PubMed: 7882171
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