| 產品名稱 |
TSHR-R5T-34 |
| 商品貨號 |
B165888 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody cross-reacts with rodent TSH receptor. The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human TSH receptor. |
| Derivation |
Spleen cells were fused with Sp2/0 mouse myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human thyroid stimulating hormone (TSH) receptor beta subunit, extracellular domain |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human thyroid stimulating hormone (TSH) receptor beta subunit, extracellular domain |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in BALB/c mice |
| Comments |
Animals were immunized with recombinant human thyroid stimulating hormone (TSH) receptor expressed in. E. coli. Spleen cells were fused with Sp2/0 mouse myeloma cells. The antibody recognizes the N-terminal extracellular domain (amino acids domain 357-389), beta subunit of the human thyroid stimulating hormone (TSH) receptor. It recognizes an extracellular epitope of the beta subunit anchored into the cellular membrane. The antibody does not interfere with hormone binding and has no effect on the receptor mediated adenylate cyclase activity. The antibody cross-reacts with rodent TSH receptor. The TSHR-R5T-34 (ATCC CRL-2683) antibody recognizes a N-terminal extracellular epitope of the beta subunit while the TSHR-T3-365 (ATCC CRL-2684) antibody recognizes a C-terminal intracellular subunit of the beta subunit. The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human TSH receptor. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10%
|
| Subculturing |
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
| Cryopreservation |
Culture medium, 45%; heat-inactivated horse serum, 45%; DMSO, 10%. |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG1; kappa light chain |
| Name of Depositor |
H Loosfelt, E Milgrom |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Baratti-Elbaz C, et al. Internalization and recycling pathways of the thyrotropin receptor. Mol. Endocrinol. 13: 1751-1765, 1999. PubMed: 10517676
de Bernard S, et al. Sequential cleavage and excision of a segment of the thyrotropin receptor ectodomain. J. Biol. Chem. 274: 101-107, 1999. PubMed: 9867816
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