| 產品名稱 |
TS2/7.1.1 |
| 商品貨號 |
B165885 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue |
spleen |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody is specific for VLA-1 alpha. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against very late antigen 1 alpha (VLA-1 alpha, integrin alpha 1); against CD49a |
| Cellular Products |
immunoglobulin; monoclonal antibody; against very late antigen 1 alpha (VLA-1 alpha, integrin alpha 1); against CD49a |
| Comments |
Animals were immunized with human cytolytic T lymphocytes. Spleen cells were fused with NS-1 myeloma cells. The antibody is specific for VLA-1 alpha. |
| Complete Growth Medium |
Culture Medium: Dulbecco's Modified Eagle's medium with 4.5 g/L glucose, 1 mM
sodium pyruvate, and 2 mM L-glutamine, 90%; fetal bovine serum, 10%. |
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml.
Interval: Maintain cell density between 5 X 10(4) and 5 X 10(5) viable cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype |
mouse IgG1 |
| Name of Depositor |
TA Springer |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Sanchez-Madrid F, et al. Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3. Proc. Natl. Acad. Sci. USA 79: 7489-7493, 1982. PubMed: 6984191
Hemler ME, et al. Glycoproteins of 210,000 and 130,000 m.w. on activated T cells: cell distribution and antigenic relation to components on resting cells and T cell lines. J. Immunol. 132: 3011-3018, 1984. PubMed: 6327814
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