| 產品名稱 |
T84.66A3.1A.1F2 [T84.66.A3.1-H-11] |
| 商品貨號 |
B165821 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension, with some loosely adherent cells |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Carcinoma |
| Applications |
The antibody reacts with CEA with an affinity constant of 2.6 X 10 exp10 l/m. The antibody reacts strongly a variety of human carcinomas and moderately with human colonic polyps. It cross-reacts weakly with granulocytes. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Animals were immunized with purified CEA. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against carcinoembryonic antigen (CEA) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against carcinoembryonic antigen (CEA) |
| Comments |
Animals were immunized with purified CEA. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with CEA with an affinity constant of 2.6 X 10 exp10 l/m. The antibody reacts strongly a variety of human carcinomas and moderately with human colonic polyps. It cross-reacts weakly with granulocytes. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.01 M HEPES and 0.01 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20%
|
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp4 viable cells/ml. Maintain cell density between 1 X 10 exp4 and 2 X 10 exp5 viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG1 kappa , IgG1; kappa light chain |
| Name of Depositor |
Beckman Research Center of City of Hope |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Crawford FG, et al. Specific hybridoma cell line and monoclonal antibodies produced from such specific hybridoma cell line and method of using such monoclonal antibodies to detect carcinoembryonic antigens. US Patent 4,873,313 dated Oct 10 1989
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