| 產品名稱 |
SW962 [SW 962, SW-962] |
| 商品貨號 |
B165789 |
| Organism |
Homo sapiens, human |
| Tissue |
vulva; Derived from metastatic site: lymph node |
| Product Format |
frozen |
| Morphology |
mixed |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Carcinoma |
| Age |
64 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Karyotype |
hypertriploid; modal number = 80; range = 70 to 85. The rate of higher ploidies was 4.3%., At least 15 marker chromosomes were common to most cells. These include: t(1p3p), t(1p17p), i(3q), t(8q9q), del(3)(p21), 20q+ and nine others. Of these, i(3q), t(8q9q), del(3)(p21) and a few others were generally paired., An additional segment on 20q+ is long, but its origin is unknown. The total length of 20q+ is as long as that of N1. Normal N3 was absent, and N1 and N18 had only a single copy per cell. Usually, there were three copies of normal X chromosomes. |
| Derivation |
The SW 962 cell line was initiated by A. Leibovitz in 1975 at the Scott and White Clinic, Temple, Texas from a surgical specimen of a lymph node metastasis from carcinoma of the vulva of a 64 year old female Caucasian. |
| Clinical Data |
The SW 962 cell line was initiated by A. Leibovitz in 1975 at the Scott and White Clinic, Temple, Texas from a surgical specimen of a lymph node metastasis from carcinoma of the vulva of a 64 year old female Caucasian. female Caucasian 64 years |
| Antigen Expression |
Blood type A; Rh+ |
| Genes Expressed |
Blood type A; Rh+ |
| Tumorigenic |
Yes |
| Effects |
Yes, in nude mice (The cells produce tumors consistent with grade I squamous cell carcinoma.) |
| Comments |
The SW 962 cell line was initiated by A. Leibovitz in 1975 at the Scott and White Clinic, Temple, Texas from a surgical specimen of a lymph node metastasis from carcinoma of the vulva of a 64 year old female Caucasian. A frozen ampule of the line at passage 14 was transferred to the ATCC in January, 1982. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: 2 to 3 times per week Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Atmosphere: air, 100% |
| STR Profile |
Amelogenin: X
CSF1PO: 10,11
D13S317: 11,14
D16S539: 9,11
D5S818: 12
D7S820: 10,11
THO1: 6,9.3
TPOX: 11
vWA: 15,17
|
| Isoenzymes |
AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 PGM1, 1-2 PGM3, 1 |
| Name of Depositor |
A Leibovitz |
| Passage History |
A frozen ampule of the line at passage 14 was transferred to the ATCC in January, 1982. |
| References |
Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034
Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474
The cells produce tumors consistent with grade I squamous cell carcinoma.
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