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SJK-237-71
SJK-237-71
規格:
貨期:
編號:B165695
品牌:Mingzhoubio

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DNA
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規格:
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產品名稱 SJK-237-71
商品貨號 B165695
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody does not react with DNA polymerases beta and gamma, and does not neutralize polymerase alpha activity.
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Antigen Expression
H-2db
Genes Expressed
immunoglobulin; monoclonal antibody; against human DNA polymerase alpha,H-2db
Cellular Products
immunoglobulin; monoclonal antibody; against human DNA polymerase alpha
Comments
Mice were immunized with fraction VIII human DNA polymerase alpha.
Spleen cells were fused with NS-1 myeloma cells.
The antibody does not react with DNA polymerases beta and gamma, and does not neutralize polymerase alpha activity.
Tested and found negative for ectromelia virus (mousepox).
Formerly ATCC TIB-178.
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
    • Subculturing Cultures can be established by addition of fresh medium or replacement of medium. Alternatively cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL. Maintain cell density between 105 and 106 viable cells/mL.
    Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
    Cryopreservation

    Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
    Culture Conditions
    Temperature: 37°C
    Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
    Isotype IgG1
    Name of Depositor D Korn
    Deposited As mouse (B cell); mouse (myeloma)
    References

    Hu SZ, et al. Preparation and preliminary characterization of monoclonal antibodies against human DNA polymerase alpha. J. Biol. Chem. 257: 8386-8390, 1982. PubMed: 7085672

    Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

    Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

    Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

    Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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