Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The hybridoma cell line R2-10F6 [R2-10F6.3C10.3F9] was established by David Presky and Victoria Wilkinson in 1993 and subcloned twice.
This antibody is a useful tool for detection of mouse IL-12p40 by ELISA and Western Blot analysis.
Derivation
The hybridoma cell line R2-10F6 [R2-10F6.3C10.3F9] was established by David Presky and Victoria Wilkinson in 1993 and subcloned twice.
Clinical Data
R2-10F6 was formed by the fusion of SP2/0 mouse myeloma cells with splenocytes from an adult female Lewis rat inoculated with recombinant mouse IL-12 p75.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse interleukin 12 (IL-12) p40
Cellular Products
immunoglobulin; monoclonal antibody; against mouse interleukin 12 (IL-12) p40
Comments
The hybridoma cell line R2-10F6 [R2-10F6.3C10.3F9] was established by David Presky and Victoria Wilkinson in 1993 and subcloned twice.
This antibody is a useful tool for detection of mouse IL-12p40 by ELISA and Western Blot analysis.
R2-10F6 was formed by the fusion of SP2/0 mouse myeloma cells with splenocytes from an adult female Lewis rat inoculated with recombinant mouse IL-12 p75.
Complete Growth Medium
Iscove's Modified Dulbecco's Medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.05 mM 2-mercaptoethanol and 100 U/ml recombinant human IL-6, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Alternately, cultures can be established by centrifugation with subsequent resuspension in fresh culture medium.
Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain at between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG2b
Name of Depositor
DH Presky
Year of Origin
1993
References
Wilkinson VL, et al. Characterization of anti-mouse IL-12 monoclonal antibodies and measurement of mouse IL-12 by ELISA. J. Immunol. Methods 189: 15-24, 1996. PubMed: 8576576
