| 產品名稱 | NCI-H358 [H-358, H358] |
|---|---|
| 商品貨號 | B165342 |
| Organism | Homo sapiens, human |
| Tissue | lung/bronchiole; derived from metastatic site: alveolus |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | bronchioalveolar carcinoma; non-small cell lung cancer |
| Gender | male |
| Applications | This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates in 1981 from tumor tissue obtained from a patient prior to initiation of chemotherapy. Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells. |
| Storage Conditions | liquid nitrogen vapor temperature |
| Images |  |
| Derivation | This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates in 1981 from tumor tissue obtained from a patient prior to initiation of chemotherapy. |
| Clinical Data | male
|
| Genes Expressed | lung surfactant associated protein A (SP-A),The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein. |
| Cellular Products | lung surfactant associated protein A (SP-A) |
| Tumorigenic | Yes |
| Effects | Yes, the cells produce tumors in athymic nude mice |
| Comments | Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells. The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein. SP-B and SP-C RNA was not expressed. They have a reported colony forming efficiency of 0.83% in soft agarose. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing | Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions | Temperature: 37°C |
| STR Profile | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 8,12 D16S539: 12,13 D5S818: 10,12 D7S820: 10,11 THO1: 6 TPOX: 8,9 vWA: 17 |
| Population Doubling Time | 38 hrs |
| Name of Depositor | AF Gazdar, JD Minna |
| Deposited As | Homo sapiens |
| Year of Origin | 1981 |
| References | Brower M, et al. Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium. Cancer Res. 46: 798-806, 1986. PubMed: 3940644 Gazdar AF, et al. Peripheral airway cell differentiation in human lung cancer cell lines. Cancer Res. 50: 5481-5487, 1990. PubMed: 2386953 NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Sordella R, et al. Gefitinib-sensitizing EGFR mutations in lung cancer activate anti-apoptotic pathways. Science 305: 1163-1167, 2004. PubMed: 15284455 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
