| 產品名稱 |
MTKP 97-12 |
| 商品貨號 |
B165204 |
| Organism |
Mus musculus, mouse |
| Tissue |
subcutaneous connective tissue; areolar and adipose |
| Cell Type |
Melanoma |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
normal |
| Age |
100 days |
| Gender |
male |
| Strain |
C3H/An |
| Applications |
produces protein antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
The MTKP 97-12 cell line was produced by transfecting LMTK- cells with the expression plasmid pMTp97b that contains a cDNA sequence coding for the p97 melanoma associated antigen. |
| Clinical Data |
male |
| Genes Expressed |
antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 [MFI2],antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 |
| Comments |
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--1.3, 2.6; HindIII--2.7; PvuII--0.6, 1.6; SstI--0.4, 1.7, 2.3; SalI--1.3. The MTKP 97-12 cell line was produced by transfecting LMTK- cells with the expression plasmid pMTp97b that contains a cDNA sequence coding for the p97 melanoma associated antigen. The cells produce approximately 4,000,000 molecules of p97 per cell under the control of the mouse metallothionein promoter. A metallothionein promoter has been added upstream of the cDNA sequence. Expression can be induced by treating with 80 uM ZnCl and 9 uM CdCl for 24 hrs. Digestion with EcoRI and HindIII excises the insert in two fragments. The larger fragment contains the complete coding sequence, that extends approximately from nt 60 to nt 2300. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium, 85%; fetal bovine serum, 15%
|
| Subculturing |
Medium Renewal: Twice per week Remove old medium, add fresh 0.25% trypsin, EDTA (0.02%) solution, rinse and remove. Allow cells to sit at room temperature (or at 37C) until cells detach (approximately 10 minutes). Add fresh medium, aspirate and dispense into new flasks. |
| Cryopreservation |
culture medium, 50%; fetal bovine serum, 40%; DMSO, 10% |
| Name of Depositor |
Oncogen |
| U.S. Patent Number |
|
| References |
Brown JP, et al. Vaccines against melanoma. US Patent 5,141,742 dated Aug 25 1992
Brian W Poor, personal communication
|
