| 產品名稱 | MG38 |
|---|---|
| 商品貨號 | B165148 |
| Organism | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue | spleen |
| Cell Type | hybridoma: B lymphocyte |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | The antibody labels mature monocyte-derived dendritic cells but shows weak or no labeling of other peripheral blood mononuclear cells. The antibody reacts with DEC-205 in tissue sections from both humans and rhesus macaque monkeys. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | DEC-205-/- knock-out mice were immunized with purified protein from human DEC-205 cells. Spleen cells were fused with Sp2/0 mouse myeloma cells. The antibody labels mature monocyte-derived dendritic cells but shows weak or no labeling of other peripheral blood mononuclear cells. |
| Genes Expressed | immunoglobulin; monoclonal antibody; against the human DEC-205 antigen (human dendritic cell antigen) |
| Cellular Products | immunoglobulin; monoclonal antibody; against the human DEC-205 antigen (human dendritic cell antigen) |
| Comments | DEC-205-/- knock-out mice were immunized with purified protein from human DEC-205 cells. Spleen cells were fused with Sp2/0 mouse myeloma cells. The antibody labels mature monocyte-derived dendritic cells but shows weak or no labeling of other peripheral blood mononuclear cells. In tissue sections, MG38 identifies DEC-205 on thymic cortical epithelium and dendritic cells in the thymic medulla and tonsillar T cell areas. The antibody reacts with DEC-205 in tissue sections from both humans and rhesus macaque monkeys. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
|
| Subculturing | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation | Freeze medium: Culture medium with an additional 5% fetal bovine serum, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype | IgG2b |
| Name of Depositor | RM Steinman |
| Deposited As | mouse (B cell); mouse (myeloma) |
| References | Guo M, et al. A monoclonal antibody to the DEC-205 endocytosis receptor on human dendritic cells. Hum. Immunol. 61: 729-738, 2000. PubMed: 10980384 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
