| 產品名稱 |
M1/9.3.4.HL.2 |
| 商品貨號 |
B165041 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Tissue |
spleen |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with an antigen, CD45, present on mouse lymph node and spleen cells but not on brain, kidney, liver or red blood cells. It antibody reacts with all isoforms of CD45. The CD45 antigen is common to all lines of leukocyte differentiation. Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Rats were immunized with C57BL/10 mouse spleen cells enriched for T lymphocytes. Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with an antigen, CD45, present on mouse lymph node and spleen cells but not on brain, kidney, liver or red blood cells. It antibody reacts with all isoforms of CD45. The CD45 antigen is common to all lines of leukocyte differentiation.
Tested and found negative for ectromelia virus (mousepox). |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse common leukocyte antigen (200000 dalton, CD45) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse common leukocyte antigen (200000 dalton, CD45) |
| Comments |
Rats were immunized with C57BL/10 mouse spleen cells enriched for T lymphocytes. Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with an antigen, CD45, present on mouse lymph node and spleen cells but not on brain, kidney, liver or red blood cells. It antibody reacts with all isoforms of CD45. The CD45 antigen is common to all lines of leukocyte differentiation.
Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium.
Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype |
rat IgG2a |
| Name of Depositor |
TA Springer |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Springer T, et al. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur. J. Immunol. 8: 539-551, 1978. PubMed: 81133
Stern PL, et al. Monoclonal antibodies as probes for differentiation and tumor-associated antigens: a Forssman specificity on teratocarcinoma stem cells. Cell 14: 775-783, 1978. PubMed: 567532
Springer TA. Monoclonal antibody analysis of complex biological systems. Combination of cell hybridization and immunoadsorbents in a novel cascade procedure and its application to the macrophage cell surface. J. Biol. Chem. 256: 3833-3839, 1981. PubMed: 7217058
Luqman M, et al. Differential expression of the alternatively spliced exons of murine CD45 in Th1 and Th2 cell clones. Eur. J. Immunol. 21: 17-22, 1991. PubMed: 1671357
Springer TACell -surface differentiation in the mouse: Characterization of Jumping and Lineage antigens using xenogeneic rat monoclonal antibodiesIn: Springer TAMonoclonal AntibodiesNew YorkPlenum Presspp. 185-217, 1980
Milstein C, et al. Monoclonal antibodies and cell surface antigens. Cell Biol. Int. Rep. 3: 1-16, 1979. PubMed: 88272
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