| 產品名稱 |
KL277 |
| 商品貨號 |
B164914 |
| Organism |
Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Animals were immunized with synthetic peptide corresponding to residues 146 to 177 of the mouse Ab/beta protein (domain beta 1). The antibody reacts with denatured beta chains of the b, d, p, q, bu and bw haplotypes. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against the beta chain of mouse Class II antigens |
| Cellular Products |
immunoglobulin; monoclonal antibody; against the beta chain of mouse Class II antigens |
| Comments |
Animals were immunized with synthetic peptide corresponding to residues 146 to 177 of the mouse Ab/beta protein (domain beta 1). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with denatured beta chains of the b, d, p, q, bu and bw haplotypes. It does not detect the intact H-2b Class II molecule, and does not detect the beta chain on the cell surface. It does not react with molecules of the a, k, f, j, s, and tl haplotypes. The reactive determinant most likely involves residues 153 through 155. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 1% nonessential amino acids, 10 mM HEPES, 0.01 mM 2-mercaptoethanol and 10% fetal bovine serum
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Atmosphere: 5% CO2 in air recommended |
| Isotype |
IgG |
| Name of Depositor |
JA Frelinger |
| Deposited As |
hamster, Armenian (B cell); mouse (myeloma) |
| References |
LaPan KE, et al. Production and characterization of a peptide specific, anti-major histocompatibility complex class II, monoclonal antibody. Mol. Immunol. 28: 499-504, 1991. PubMed: 1648172
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