| Applications |
The hybridoma cell line JAA-F11 was established in 1995. The hybridoma produces an antibody (IgG3) that binds the saccharide antigen, Gal beta1-3GalNAc (T-antigen). The antibody binds to breast adenocarcinoma tissue but not to normal tissues. It may be useful in tumor studies, germinal center studies and T cell maturation studies. The JAA-F11 cell line was formed by the fusion of P3X63Ag8.653 mouse myeloma cells with splenocytes from a BALB/c mouse inoculated with a synthetic Gal beta1-3GalNAc-BSA conjugate. |
| Comments |
The hybridoma cell line JAA-F11 was established in 1995. The hybridoma produces an antibody (IgG3) that binds the saccharide antigen, Gal beta1-3GalNAc (T-antigen). The saccharide antigen, Gal beta1-3GalNAc is of biologic importance in many systems. It is a tumor-associated carbohydrate antigen, a temporally expressed antigen in germinal center B cells and cortical T cells, a parasite-associated antigen, a spermatozoa vitality marker and an antigen on aged red blood cells. The antibody binds to breast adenocarcinoma tissue but not to normal tissues. It may be useful in tumor studies, germinal center studies and T cell maturation studies. The JAA-F11 cell line was formed by the fusion of P3X63Ag8.653 mouse myeloma cells with splenocytes from a BALB/c mouse inoculated with a synthetic Gal beta1-3GalNAc-BSA conjugate. |
| References |
Rittenhouse-Diakun K, et al. Development and characterization of monoclonal antibody to T-antigen: (gal beta1-3GalNAc-alpha-O). Hybridoma 17: 165-173, 1998. PubMed: 9627057
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
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