| 產(chǎn)品名稱 |
J26 |
| 商品貨號 |
B164873 |
| Organism |
Mus musculus, mouse |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
100 days |
| Gender |
male |
| Strain |
C3H/An |
| Applications |
This line was derived from the L-M(TK-) cell line by transformation with human genomic DNA and a plasmid containing the Herpes simplex virus thymidine kinase gene and then selecting for transformants positive for beta 2 microglobulin. The cells can be used as recipients for the the transfer of individual HLA genes. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
This line was derived from the L-M(TK-) cell line by transformation with human genomic DNA and a plasmid containing the Herpes simplex virus thymidine kinase gene and then selecting for transformants positive for beta 2 microglobulin. |
| Clinical Data |
male |
| Genes Expressed |
human beta-2-microglobulin (beta 2 microglobulin) |
| Cellular Products |
human beta-2-microglobulin (beta 2 microglobulin) |
| Comments |
This line was derived from the L-M(TK-) cell line by transformation with human genomic DNA and a plasmid containing the Herpes simplex virus thymidine kinase gene and then selecting for transformants positive for beta 2 microglobulin. The cells can be used as recipients for the the transfer of individual HLA genes. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 0.03 mM hypoxanthine, 0.001 mM amethopterin and 0.02 mM thymidine, 90%; fetal bovine serum, 10%
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: 2 to 3 times per week Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Name of Depositor |
P Kavathas |
| Deposited As |
Mus musculus |
| References |
Kavathas P, Herzenberg LA. Stable transformation of mouse L cells for human membrane T-cell differentiation antigens, HLA and beta 2-microglobulin: selection by fluorescence-activated cell sorting. Proc. Natl. Acad. Sci. USA 80: 524-528, 1983. PubMed: 6188154
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