| 產品名稱 |
GL7 |
| 商品貨號 |
B164508 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with a 35000 dalton antigen present on subsets of activated mouse B cells and activated CD4 + and CD 8 + mouse T cells. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse lymphocytes |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse lymphocytes |
| Comments |
Animals were immunized with LPS activated DBA/2J mouse B cells. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with a 35000 dalton antigen present on subsets of activated mouse B cells and activated CD4 + and CD 8 + mouse T cells. The GL7 antigen is expressed on up to 70% of activated B cells and on high proportions of ConA activated CD4 + and CD8 + T cells. Unstimulated B and T cells are infrequently GL7 positive. The antigen appears to be a lymphocyte activation antigen that is different from those previously identified. |
| Complete Growth Medium |
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine, 1 mM sodium pyruvate,
1% nonessential amino acids and 50 ?M 2-mercaptoethanol, 90%; fetal bovine
serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgM |
| Name of Depositor |
G Lazlo, KS Hathcock |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Laszlo G, et al. Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J. Immunol. 150: 5252-5262, 1993. PubMed: 8515058
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