| 產品名稱 |
Gekko lung-1 |
| 商品貨號 |
B164500 |
| Organism |
lizard, gecko (Gekko gecko), lizard, gecko |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
adult |
| Gender |
male |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
modal number = 38; range = 34 to 135.
Bimodal stemline number of 37 and 38 chromosomes. Stable pseudodiploid karyotype in 38 chromosome stemline number. The change from normal is a constant deletion in one of the largest chromosomes. Slightly unstable karyology in 37 chromosome stemline number. |
| Clinical Data |
male |
| Virus Susceptibility |
Vaccinia virus
Vesicular stomatitis, Orsay (Indiana)
Human poliovirus 1
|
| Complete Growth Medium |
Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
|
| Subculturing |
Protocol: - Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 30°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: Two to three times per week |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 30.0°C |
| Name of Depositor |
HF Clark |
| Deposited As |
lizard, gekko |
| Year of Origin |
June, 1965 |
| References |
Cohen MM, et al. The somatic chromosomes of 3 lizard species: Gekko gecko, Iguana iguana, and Crotaphytus collaris. Experientia 23: 769-771, 1967. PubMed: 6062904
Clark HF, et al. Characterization of reptilian cell lines established at incubation temperatures of 23 to 36 degrees. Proc. Soc. Exp. Biol. Med. 133: 1039-1047, 1970. PubMed: 4314147
Cohen MM, Clark HF. Spontaneous chromosomal alteraion in cell lines of poikilothermic origin (Gekko gecko). Cytogenetics 7: 16-26, 1968. PubMed: 5659169
|
