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CL18/6
CL18/6
規格:
貨期:
編號:B164264
品牌:Mingzhoubio

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產品名稱 CL18/6
商品貨號 B164264
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody reacts with canine intercellular adhesion molecule 1 (ICAM-1).
Antibody produced by CL18/6 (ATCC CRL-2518), but not CL18/1 (ATCC CRL-2517), blocks adherence of canine neutrophils to lipopolysaccharide (LPS) stimulated canine jugular vein endothelial cells.
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
Antibody produced by CL18/6 (ATCC CRL-2518), but not CL18/1 (ATCC CRL-2517), blocks adherence of canine neutrophils to lipopolysaccharide (LPS) stimulated canine jugular vein endothelial cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against canine intracellular adhesion molecule 1 (ICAM-1).
Cellular Products
immunoglobulin; monoclonal antibody; against canine intracellular adhesion molecule 1 (ICAM-1).
Comments
Animals were immunized with canine endothelial cells that had been stimulated with lipopolysaccharide (LPS).
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
The antibody reacts with canine intercellular adhesion molecule 1 (ICAM-1).
Antibody produced by CL18/6 (ATCC CRL-2518), but not CL18/1 (ATCC CRL-2517), blocks adherence of canine neutrophils to lipopolysaccharide (LPS) stimulated canine jugular vein endothelial cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Maintain cell density between 5 x 104 and 1 x 106 viable cells/mL.

Medium Renewal:  Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG1
Name of Depositor C Smith
Deposited As mouse (B cell); mouse (myeloma)
References

Smith CW, et al. Adherence of neutrophils to canine cardiac myocytes in vitro is dependent on intercellular adhesion molecule-1. J. Clin. Invest. 88: 1216-1223, 1991. PubMed: 1680878

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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