| 產品名稱 | CCRF-SB [CCRF SB] |
|---|---|
| 商品貨號 | B164219 |
| Organism | Homo sapiens, human |
| Tissue | peripheral blood |
| Cell Type | B lymphoblast |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension |
| Biosafety Level | 2 [Cells contain Herpesvirus]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | acute lymphoblastic leukemia |
| Age | 11.5 years |
| Gender | Male |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | Derived from a buffy coat preparation from a patient with acute lymphoblastic leukemia. A T cell line (HSB-2, ATCC CCL-120.1) was also obtained from the same buffy coat preparation. |
| Clinical Data | 11.5 years
Caucasian
male |
| Antigen Expression | HLA A1, A2, B12, B17, Cw2 |
| Comments | The cells are EBNA positive |
| Complete Growth Medium | RPMI 1640 medium, 90%; fetal bovine serum, 10% - OR - Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
|
| Subculturing | Cultures can be maintained by addition or replacement of medium. Establish new cultures at 3 X 105 viable cells/mL.
Medium Renewal: Add fresh medium (10% to 20% by volume) every 2 to 3 days |
| Cryopreservation | culture medium 95%; DMSO, 5% |
| Culture Conditions | Temperature: 37°C |
| STR Profile | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 10,12 D16S539: 9,13 D5S818: 11,12 D7S820: 11,12 THO1: 9,10 TPOX: 8 vWA: 18 |
| Isoenzymes | G6PD, B |
| Name of Depositor | GE Foley |
| Deposited As | Homo sapiens |
| References | Foley GE et al. M.D. Anderson Hospital and Tumor Inst. Monograph 21, 1967. Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967. PubMed: 4170381 Santra M, et al. Ectopic expression of decorin protein core causes a generalized growth suppression in neoplastic cells of various histogenetic origin and requires endogenous p21, an inhibitor of cyclin-dependent kinases. J. Clin. Invest. 100: 149-157, 1997. PubMed: 9202067 Qian JX, et al. Decreased interleukin-15 from activated cord versus adult peripheral blood mononuclear cells and the effect of interleukin-15 in upregulating antitumor immune activity and cytokine production in cord blood. Blood 90: 3106-3117, 1997. PubMed: 9376592 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
