| 產品名稱 |
A375.S2 |
| 商品貨號 |
B163876 |
| Organism |
Homo sapiens, human |
| Tissue |
skin |
| Cell Type |
Melanoma |
| Product Format |
frozen |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
malignant melanoma |
| Age |
54 years |
| Gender |
female |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
This line was cloned by limiting dilution from the A375 melanoma cell line (see ATCC CRL-1619). |
| Clinical Data |
female |
| Tumorigenic |
Yes |
| Effects |
Yes, in immunosuppressed mice |
| Comments |
These cells will eventually die and lyse when cultured in medium containing interleukin-1 (interleukin 1, IL-1) and therefore can be used in a reliable and sensitive growth inhibition assay for IL-1.
Sensitivity to IL-1 is maximal when the cells are derived from subconfluent cultures in logarithmic growth. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove medium, rinse with fresh 0.25% trypsin, 0.03% EDTA solution and let the culture sit at room temperature (or 37°C) until cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 11,12 D13S317: 11,14 D16S539: 9 D5S818: 12 D7S820: 9 THO1: 8 TPOX: 8,10 vWA: 16,17 |
